Academic CentreLaboratory of Environment Genetics
Equipment for DNA amplification and analysis Equipment for plant tissue culture International cooperation Agricultural Research Institute Krom???ž, Ltd., Czech Republic Research Institute for Crop Production, Czech Republic Lithuanian Institute of Agriculture Institute of Ecology, Vilnius University, Lithuania Plant Gene Bank of Lithuania Jõgeva Plant Breeding Institute, Estonia N.I. Vavilov Research Institute of Plant Industry (VIR), Russia Research Institute of Plant Production Pieš?any, Slovakia International Plant Genetic Resources Institute, Italy Nordic Gene Bank, Sweden Swedish University of Agricultural Sciences, Alnarp, Sweden Université de Picardie, Amiens, France Université de Reims, Reims, France Projects 2015-2009 1. European co-operation in the field of scientific and technical research: “Quality Enhancement of Plant Production through Tissue Culture” COST Action 843 (2004-2005). 2. European co-operation in the field of scientific and technical research: „Genetic determination of the embryogenesis and organogenesis in cereals for the optimizing of double haploid production” COST Action 841 (2005-2008). 3. Latvian Council of Science project: „Detection of adaptive genes and their mapping by means of molecular markers in the barley varieties of the different origin” (2005-2008). 4. Project “Latvian field crop diversity evaluation and breeding optimisation by modern biotechnology methods” of the State Research Programme “Innovative technologies for high quality, safe and healthy food production from genetically, physiologically and biochemically diverse plant and animal raw material” (2006–2009). 5. Latvian Council of Science project: Creating of well adapted crop varieties and their maintenance breeding by using traditional and biotechnological methods in conventional and organic farming (2006-2009). 6. Latvian Council of Science project: Adaptive changes of genetic polymorphism of cultivated plants under selection and environment influence (2009-2012). 7. Latvian Council of Science Project: Investigation of Latvian renewable raw materials – flax and hemp products for development of innovative technologies and new functional materials. izstrādei (2010-2014). 8. Lithuanian-Latvian- Taiwan (Peopleis Republic of China) Collaboration Project: Evaluation of nuclear power impact on perch (Perca fluviatilis) population by means of genetic based analysis and application of fish otoliths as natural recorders of radiocontamination and thermal polution (2010-2013) 9. European Regional Development Fund Project 2010/0321/2DP/2.1.1.1.0/10/APIA/VIAA/144 "Plant breeding technology development on the biocompatible microchips" (2011-2014). 10. Valsts pētījumu programmas „Latvijas ekosistēmu vērtība un tās dinamika klimata ietekmē (EVIDEnT) projekts Nr.4.4 „Latvijas reto un apdraudēto sugu ģenētisko īpatnību izpēte”. (vad. Ī. Rašals, 2015.- 2017.). Patents: 1. Spring barley variety ‘AUSTRIS’; SPP State Stende Cereals Breeding Institute; NR. MV-31; 24/11/2009. 2. Spring wheat variety 'ROBIJS‘; State Stende Cereals Breeding Institute NR. KVV-14; 12/05/2011. Publications KRASŅEVSKA, N., GRAUDA, D., KĻAVIŅA, D. and RASHAL, I., 2017. Endopolyploidy of endangered plant species ligularia sibirica in different environments, Vide. Tehnologija. Resursi - Environment, Technology, Resources2017, pp. 161-164. STAFECKA, I., GRAUDA, D. and STRAMKALE, V., 2017. Diseases diversity for flax genetic resources in Latvia, Vide. Tehnologija. Resursi - Environment, Technology, Resources 2017, pp. 278-283. STAFECKA, I., STRAMKALE, V. and GRAUDA, D., 2016. Estimation of yield stbility for flax genetic resource using regression and cluster analysis. ReseaRch foR RuRal Development, 1. Book Series: Research for Rural Development. p. 15-22. GRAUDA, D., BUMBURE, L., LYASHENKO, I., KATASHEV, A., DEKHTYAR, Y. and RASHAL, I., 2015. Amber particles as living plant cell markers in flow cytometry. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 77-81. GRAUDA, D., KOLODYNSKI, A., BELOGRUDOVA, I., BUMBURE, L. and RASHAL, I., 2015. Combined effects of 50Hz electromagnetic field and SiO2 nanoparticles on oxidative stress in plant's gametic cells. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 82-86. LICITE, V., BOIKOVA, E., BOTVA, U., GRAUDA, D. and BUHOLCE, L., 2015. Microbial food web components as potential indicators of urban hydroecosystems. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 98-104. GRAUDA, D., AVOTIŅŠ, K., FOKINA, O., KOLODINSKA-BRANTESTAM, A. and RASHAL, I., 2015. Genetic diversity of white clover (Trifolium Repens L.) from the Urban Area of Riga. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 132-134. KOLODINSKA-BRANTESTAM, A., BOIKO, D., GRAUDA, D., KRASŅEVSKA, N. and RASHAL, I., 2015. Genetic diversity of mute swan population of the Riga Urban Area. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 135-139. GRAUDA, D. and ČEKSTERE, G., 2015. Current investigations of urban enviromental quality in Latvia. Proceedings of the Latvian Academy of Sciences. Section B, 69(3), pl. A1. CEKSTERE, G., KARLSONS, A. and GRAUDA, D., 2015. Salinity-induced responses and resistance in Trifolium repens L. Urban Forestry and Urban Greening, 14(2), pp. 225-236. FOKINA, O., GRAUDA, D. and RASHAL, I., 2015. Genetic diversity of two perch Perca fluviatilis populations of the Latgale region, Vide. Tehnologija. Resursi - Environment, Technology, Resources 2015, pp. 96-98. GRAUDA, D., RASHAL, I., BELOGRUDOVA, I., KATASHEV, A. and BUMBURE, L., 2015. Influence of SiO2 nanoparticles on relative fluorescence of plant cells, Vide. Tehnologija. Resursi - Environment, Technology, Resources2015, pp. 107-111. STAFECKA, I., STRAMKALE, V. and GRAUDA, D., 2015. Yield development of flax varieties and lines within variable environment in Latvia, Vide. Tehnologija. Resursi - Environment, Technology, Resources 2015, pp. 297-302. LEPSE, L., RASHAL, I. and AKA-KACAR Y., 2014. Assessment of genetic variability between inbred and siblines of Latvian origin cucumber population using RAPD markers. G. Sarkisyan (Ed.) Proc. Eurasian Symposium on Vegetables and Greens. Acta Horticulturae, 1033, pp. 31-38. KĻAVIŅA, D., GRAUDA, D., PRIEDE, A. and RASHAL, I., 2014. Habitat diversity and genetic variability of Cypripedium calceolus in Latvia. Proceedings of 6th Planta Europa Conference, Committee on Nature Conservation, Polish Academy of Sciences, Kraków, pp. 91–97. RAGAUSKAS, A. BUTKAUSKAS, D. SRUOGA, A. KESMINAS, V. RASHAL, I. and TZENG, WN., 2014. Analysis of the genetic structure of the European eel Anguilla anguilla using the mtDNA D-loop region molecular marker. Fisheries Science, 80(3), pp. 463-474. GRAUDA, D., LAPIŅA, L., JANSONE, B., JANSONS, A. and RASHAL, I., 2013. Recovering genetic resources of some legume species of Latvian origin by plant tissue culture. Proceedings of the Latvian Academy of Sciences. Section B, 67(3), pp 224-228. KOKINA, I., GAVARANE, I. and RASHAL, I., 2013. Results of the long-termobservation of the population of Blumeria graminis f.sp hordei in Latvia and Lithuania. FEBS JOURNAL 280, pp. 558-558. GRAUDA, D. MIKELSONE, A. AUZINA, A. STRAMKALE, V. and RASHAL, I., 2013. Use of Plant Biotechnology Methods for flax breeding in Latvia. In: Zaikov G. E., Pudel F., eds. Organic Chemistry, Biochemistry, Biotechnology and Renewable Resources. Research and Development. Vol. 1. Today and Tomorrow. Nova Science Publishers, Inc., USA, pp. 1-10. BLEIDERE, M., GRUNTE, I., GRAUDA, D. and RASHAL, l., 2012. Morphological and agronomic characterizion of spring barley variety ‘Austris’., Acta Biol. Univ. Daugavpiliensis, 12(1), pp 44-50. LIN, YJ., SHIAO, JC., PLIKSHS, M., MINDE, A., LIZUKA, Y., RASHAL, I. and TZENG, WN., 2012. Otolith Sr:CA Ratios as a Natural Mark to Discriminate Restocked and Naturally Recruited European Eels in Latvia. Advances in fish tagging and marking tehnology. Book series: American Fisheris society symposium. 76, pp.. 439-452. BELOGRUDOVA, I., GRAUDA, D., JAKOBSONE, G. and RRASHAL, I., 2012. Usability of retrotransposone-based molecular marker system to assess genetic diversity of Liparis loeselii., Acta Biol. Univ. Daugavpiliensis, 12(1), pp 40-43. LAPIŅA, L., GRAUDA, D. and RASHAL, I., 2012. Using retrotransposon-based molecular markers for analysis of genetic variability in Latvian populations of alfalfa. Acta Biol. Univ. Daugavpiliensis, 12(1), pp 84-88. BUTKAUSKAS, D., RAGAUSKAS, A., SRUOGA, , KESMINAS, V., LOŽYS, L., RASHAL, , TZENG, W.-N. and ŽALAKEVIČIUS, M., 2012. Investigations into genetic diversity of the perch inhabiting Ignalina nuclear power plant cooler and other inland water bodies of Lithuania on the basis of mtDNA analysis., Veterinarija ir Zootchnika, 60(82), 7-15. KOKINA, I. and RASHAL, I., 2012. Results of monitoring of the population of Blumeria graminis f.sp. hordei in Latvia in 2009-2010., Proc. Latvian Acad.Sci., Sect. B, 66(1/2), pp 41-47. KOLODINSKA BRANTESTAM, A., RASHAL, I., TUVESSON, S., WEIBULL, J.and R. VON BOTHMER, 2012. Genetic profiles and diversity of baltic spring barley material., Proc.Latvian Acad. Sci,, Sect.B, 66(1/2), pp 10-20. KĻAVIŅA, D. GRAUDA, D. PRIEDE, A. and RASHAL, I., 2011. A complex investigation of Cypripedium calceolus populations in Latvia. Planta Europa, pp 10. GRAUDA, D., LEPSE, N., STRAZDIŅA, V., KOKINA, I., LAPIŅA, L., MIĶELSONE, A., ĻUBINSLIS, L. and RASHAL I., 2010. Obtaining of doubled haploid lines by anther culture method for the Latvian wheat breeding. Agronomy Research, 8 ( 3), pp. 545-552. GRAUDA, D., RASHAL, I. and STRAMKALE, V., 2006. The Use In vitro Methods for Obtaining Flax Breeding Source Material. In: “Renewable Resources and Plant Biotechnology”, Eds. R. Kozlowski, G. E. Zaikov, F. Pudel. Nova Publishers, pp. 127-134. GRAUDA, D., LEGZDIŅA, L. and RASHAL, I., 2007. Use of somaclonal variation for obtaining of barley breeding source material. Zemdirbyste-Agriculture, 94(4), pp. 105-110. LĀCIS, G., KAUFMANE, E., RASHAL, I., TRAJKOVSKI, V. and IEZZONI, A.F., 2008. Identification of self-incompatibility (S) alleles in Latvian and Swedish sweet cherry genetic resources collections by PCR based typing. Euphytica, 160 (2), pp. 155.–163. KOKINA, I. and RASHAL I., 2008. Results of the monitoring of the population of Blumeria graminis f.sp. hordei in the Latgale region of Latvia in 2007. Zemdirbyste-Agriculture, 95(3), pp. 320-326. GARKAVA-GUSTAVSOON, L., KOLODINSKA BRANTESTAM, A., SEHIC, J. and NYBOM. H., 2008. Molecular characterisation of indigenous Swedish apple cultivars based on SSR and S-allele analysis. 2008. DOI: 10.1111/j.0018-0661.2008.2042.x SRUOGA, A., BUTKAUSKAS, D. and RASHAL I., 2008. Evaluation of genetic diversity of perch (Perca fluviatilis) and pikeperch (Sander lucioperca) populations from Curonian Lagoon and inshore waters of the Baltic Sea. Acta Biologica Universitatis Daugavpiliensis, 8(1), pp. 81-88. GRAUDA, D., MIĶELSONE, A. and RASHAL I., 2009. Use of antioxidants for enhancing flax multiplication rate in tissue culture. Acta Horticulturae, 812, pp. 147-151. LĀCIS, G., RASHAL I., RUISA, S., TRAJKOVSKI, V. and IEZZONI, A.F., 2009. Assessment of genetic diversity of Latvian and Swedish sweet cherry (Prunus avium L.) genetic resources collections by using SSR (microsatellite) markers. Sci. Hortic., 121, pp. 451-457. LAPIŅA, L., GRAUDA, D.,JANSONE, B., JANSONS, A. and RASHAL I., 2009. Restoration of Latvian alfalfa (Medicago sativa) genetic resources perspective for breeding. Proceedings of the 7th International Scientific and Practical Conference “Environment. Technology. Resources”, Rēzekne, Latvia, June 25-27, 2009. Vol. 1. Rēzekne, pp. 166-168. JACGUARD, C., NOLIN, F., HECART, D., GRAUDA, D., RASHAL, I., DHONDT-CORDELIER, S., SANGVAN,R.S., DEVAUX, P., MAZREYRAT-GOUBEYRE, F. and CLEMENT, C., 2009. Microspore embryogenesis and programmed cell death in barley: effects of copper on albinism in recalcitrant cultivars. Plant Cell Reports, 28, 1329–1339. KOLODINSKA BRANTESTAM, A., von BOTHMER, R., RASHAL, I., GULLORD, M., MARTYNOV, S, and WEIBULL, I., 2010. Variation of agronomic traits in Nordic and Baltic spring barley. Proceedings of the 10th International Barley Genetics Syposium. Alexandria, Egypt, April 5-10, 2008. Eds. S. Ceccarelli, and S. Grando. Proceedings of the 10th International Barley Genetics Symposium, 5-10 April 2008, Alexandria, Egypt. ICARDA, Aleppo, Syria, pp. 39-45. LĀCIS, G., RASHAL, I., and TRAJKOVSKI, V., 2010. Comparative analysis of sweet cherry (P. avium) genetic diversity revealed by two methods of SSR marker detection. Proceedings of the Latvian Academy of Sciences, Section B, 64(3/4), pp. 149-158. SKRABULE, I., GRAUDA, D., MIKELSONE, A. and VASARIETE, A., 2010. Adaptation of glycoalkoids detection method for evaluation of Latvian potato genetic resources. Agronomy Research, 8, pp. 705-711. LĀCIS, G., TRAJKOVSKI, V., and RASHAL, I., 2010. Phenotypical variability and genetic diversity within accessions of the Swedish sour cherry (Prunus cerasus L.) genetic resources collection. Biologija, 56(1/4), pp. 1-8. LĀCIS, G., RASHAL, I., and TRAJKOVSKI, V., 2010. Implementation of a limited set of SSR markers for screening of genetic variability in Latvian and Swedish sour cherry (Prunus cerasus L.) genetic resources collections. Proceedings of the Latvian Academy of Sciences. Section B, 65(1/2), pp. 21-28. KOLODINSKA BRANTESTAM, A., von BOTHMER, R., RASHAL, I. and WEIBULL, I., 2003. Changes in the genetic diversity of barley of Nordic and Baltic origin, studied by isozyme electrophoresis. Plant Genetic Resources: Characterization and Utilization, 1(2/3), pp. 143-149. DREISEITL, A. and RASHAL, I., 2004. Powdery mildew resistance genes in Latvian barley varieties. Euphytica, 135(3), pp. 325-332. KOLODINSKA BRANTESTAM, A., von BOTHMER, R., DAYTEG, C., RASHAL, I., TUVESSON, S.and WEIBULL, I., 2004. Inter simple sequence repeat analysis of genetic diversity and relationships in cultivated barley of Nordic and Baltic origin. Hereditas, 141(2), pp. 186-192. GRAUDA, D., KEIŠA, A. and RASHAL, I. 2005. Obtaining of doubled haploid lines for Latvian barley and wheat breeding programs by anther culture method. In: K. Annamaa (ed.). Plant Breeding and Seed Science, IX. Jõgeva, Jõgeva Plant Breeding Institute, pp. 209-216. KOKINA, I. and RASHAL I., 2006. Monitoring the population of Blumeria graminis f.sp. hordei in the South-Eastern part of Latvia. Agronomy Research, 4, pp. 231-236.