Students and staff

Laboratory of Environment Genetics

Laboratory of Environment Genetics

Address:Jelgavas Street 1, Riga
E-mail: dace.grauda@lu.lv
Head: Dr. biol. Dace Grauda

Research directions

Conservation and research of crop genetic resources
Development of crop selection material using traditional and biotechnological methods
Establishment of DH lines for barley, flax and other crops using and developing microspores in vitro
Determination of Latvian barley adaptive gene complex and molecular mapping
Latvian population of barley meal Blumeria graminis f.sp. hordei monitoring
Identification and use of new barley powdery mildew resistance donors

Equipments

Equipment for plant DNA research
Equipment for tissue, microspore, pollen culture in vitro experiments
Restoration of seed germination by in vitro tissue culture
Lab Insights www.youtube.com/watch

International cooperation

  • Agricultural Research Institute in Kromeriz, Czech Republic
  • Institute of Cereal Production, Czech Republic
  • Institute of Agriculture, Lithuania
  • Institute of Ecology, Vilnius University, Lithuania
  • Plant Gene Bank, Lithuania
  • Jogeva Plant Breeding Institute, Estonia
  • N.I. Vavilov Crop Research Institute (VIR), Russia
  • Crop Research Institute, Piešťany, Slovakia
  • Nordic Gene Bank, Sweden University of Sweden, Alnarpa, Sweden 
  • University of Picardy, Amiens, France
  • University of Reims, Reims, France

State Research Program

“Value of Latvian Ecosystems and Its Dynamics under the Influence of Climate (EVIDEnT)“ Project No. 4.4 "Research of Genetic Peculiarities of Latvia's Rare and Endangered Species”(2014-2017)

Projects

  • Ifsitex!http://www.jlutechnologies.lv/index.php?option=com_content&view=article&id=167:eureka-network-project-innovation-across-borders-&catid=1:latest-news&lang=en&Itemid=
  • Test 4 SME https://projects.interreg-baltic.eu/projects/test-4-sme-107.html
  • European Regional Development Fund Project 2010/0321/2DP/2.1.1.1.0/10/APIA/VIAA/144 "Plant breeding technology development on the biocompatible microchips" (2011-2014).
  • Latvian Council of Science Project: Investigation of Latvian renewable raw materials – flax and hemp products for development of innovative technologies and new functional materials. (2010-2014).
  • Lithuanian-Latvian- Taiwan (Peopleis Republic of China) Collaboration Project: Evaluation of nuclear power impact on perch (Perca fluviatilis) population by means of genetic based analysis and application of fish otoliths as natural recorders of radiocontamination and thermal polution (2010-2013)
  • Latvian Council of Science project: Adaptive changes of genetic polymorphism of cultivated plants under selection and environment influence (2009-2012)
  • Project “Latvian field crop diversity evaluation and breeding optimisation by modern biotechnology methods” of the State Research Programme “Innovative technologies for high quality, safe and healthy food production from genetically, physiologically and biochemically diverse plant and animal raw material” (2006–2009).
  • Latvian Council of Science project: Creating of well adapted crop varieties and their maintenance breeding by using traditional and biotechnological methods in conventional and organic farming (2006-2009).
  • European co-operation in the field of scientific and technical research: „Genetic determination of the embryogenesis and organogenesis in cereals for the optimizing of double haploid production” COST Action 841 (2005-2008).
  • Latvian Council of Science project: „Detection of adaptive genes and their mapping by means of molecular markers in the barley varieties of the different origin” (2005-2008).
  • European co-operation in the field of scientific and technical research: “Quality Enhancement of Plant Production through Tissue Culture” COST Action 843 (2004-2005)..

GRAUDA, D., KATAŠEVS, A., BELOGRUDOVA, I., ... ŽAGATA, K., RASHAL, I. 2024. Influence of SiO2 nanoparticles on gametic cells of lime trees from urban area detected by flow cytometry. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 78(5-6), 362–371.

IGNATAVIČIENĖ, I., VYŠNIAUSKIENĖ, R., RANČELIENĖ, V., ... GRAUDA, D., BUTKAUSKAS, D. 2024. The effects of electromagnetic field radiation of extremely low frequency on growth parameters and nucleotide substitutions in L. minor clones. Acta Physiologiae Plantarum,  46(4), 47.

BIRBELE, E., DI MARZIO, A., GRAUDA, D., VIMERCATI, G., DEKSNE, G. 2024. Genetic diversity of European tree frogs (Hyla arborea group): a systematic review. European Journal of Ecology, 10(1), 1–16.

SANCHANIYA, J.V., LASENKO, I., VIJAYAN, V., SMOGOR, H., GOBINS, V., KOBEISSI, A., GOLJANDIN, D. 2024. A novel method to enhance the mechanical properties of polyacrylonitrile nanofiber mats: an experimental and numerical investigation. Polymers, 2024, 16(7), 992.

ANCHANIYA, J.V., LASENKO, I., GOBINS, V., KOBEISSI, A., GOLJANDIN, D. 2024. A finite element method for determining the mechanical properties of electrospun nanofibrous mats. Polymers, 2024, 16(6), 852.

RAGAUSKAS, A., IGNATAVIČIENĖ, I., RAKAUSKAS, V., GRAUDA, D., PRAKAS, P., BUTKAUSKAS, D. 2023. Trends of Eurasian perch (Perca fluviatilis) mtDNA ATP6 region genetic diversity within the hydro-systems of the eastern part of the Baltic Sea in the Anthropocene. Animals, 2023, 13(19), 3057.

GRAUDA, D., BUTKAUSKAS, D., VYŠNIAUSKIENĖ, R., ... ŽAGATA, K., GOBIŅŠ, V., RASHAL, I., VILCĀNE, I., ĻAŠENKO, I. 2023.  Establishment of Biotesting System to Study Features of Innovative Multifunctional Biotextile. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 2023, 77(3-4), 186–192.

SANCHANIYA, J.V., LASENKO, I., KANUKUNTALA, S.P., ... TIPANS, I., GOBINS, V. 2023. Mechanical and thermal characterization of annealed oriented PAN nanofibers. Polymers, 2023, 15(15), 3287.

SANCHANIYA, J.V., LASENKO, I., KANUKUNTLA, S.P., ... VILUMA-GUDMONA, A., GOBINS, V. 2023. Preparation and characterization of non-crimping laminated textile composites reinforced with electrospun nanofibers. Nanomaterials, 13(13), 1949.

ŽAGATA, K., BUTKAUSKAS, D., VYŠNIAUSKIENĖ, R., ... RASHAL, I., GRAUDA, D. Establishing of an axenic Lemna minor Line as a model organism for a wide spectre of biological and environmental investigations. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 77(3-4), 178–185.

LASENKO, I., GRAUDA, D., BUTKAUSKAS, D., ... VILUMA-GUDMONA, A., LUSIS, V. 2022. Testing the Physical and Mechanical Properties of Polyacrylonitrile Nanofibers Reinforced with Succinite and Silicon Dioxide Nanoparticles. Textiles, 2(1), 162–173.

MOROZOVA, I., GRAUDA, D., STRAMKALE, V., KROICA, I., STRAMKALIS, A. 2022. Assessment of flax population productivity under variable ecological factors during ontogenesis. Acta Biologica Universitatis Daugavpiliensis, 22 (2), 157–165.

KRASŅEVSKA, N., MIĶELSONE, A., KRUCHONOK, A., RASHAL, I., BUTKAUSKAS, D., GRAUDA, D. 2021. Assessment of IPBs primers potential to be used in genetic diversity studies of wild cloudberry (Rubus chamaemorus L.) populations. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences,  76 (2), 314–316.

Ragauskas, A., Butkauskas, D., Prakas, P., Gadliauskienė, K., Gajduchenko, H., Grauda, D., 2020. Complex phylogeographic relationships among the Eurasian perch (Perca fluviatilis) populations in the eastern part of the Baltic Sea Region. Hydrobiologia, 847 (3), 925–938. (Q1 žurnāls)

Rebāne, A., Grauda, D., Rancāne, S., Jansons, A., Rashal, I., 2020. Use of flow cytometry for producing tetraploids in red clover. Proceedings of the Latvian Academy of Sciences. Section B, 74 (5), 344–346.

Fokina, O., Grauda, D., Puriņa, I., Bārda, I., Rashal, I., 2020. Genetic structure of the Limecola balthica population in the Gulf of Riga, Baltic Sea. Proceedings of the Latvian Academy of Sciences. Section B, 74 (6), 381–384.

Butkauskas, D., Starodubaitė, M., Potapov, M., Potapova, O., Abramov, S., Litvinov, Y., 2020. Phylogenetic relationships between zokors Myospalax (Mammalia, Rodentia) determined on the basis of morphometric and molecular analyses. Proceedings of the Latvian Academy of Sciences. Section B, 74 (1), 25–34.

Butkauskas, D., Bea, A., Yanenko, V., Prakas, P., Švažas, S., Vaitkuvienė, D., 2020. MtDNA D-loop genetic diversity of common quail (Coturnix coturnix) through Ukraine and Spain. Turkish Journal of Zoology, 44 (5), 477–480.

Žagata, K., Grauda, D., Rashal, I., 2020. Effectiveness of doubled haploids production by anther culture from selected winter wheat hybrids. Acta Biologica Universitatis Daugavpiliensis, 20 (2), 107 – 111.

Butkauskas, D., Grauda, D., Vysniauskiene, R., Ranceliene, V., Ignataviciene, I., 2020. Identification and Application of Informative Genetic Markers in studies of Genetic Diversity of Experimental and Wild Populations of Lemna minor. 78th International Scientific Conference of the University of Latvia, Innovative and applied research in Biology, Proceedings, 2, 7-9.

Grauda, G., Butkauskas, D., Vysniauskiene, R., Ranceliene, V., Ragauskas, A., Jankevica, L., Krasņevska, N., Miķelsone, A., Žagata, K., Ažēna, E., Selga, T., Gobiņš, V., Dubova, I., Lashenko, I., 2020. Development of methods for biotextile testing. 78th International Scientific Conference of the University of Latvia, Innovative and applied research in Biology, Proceedings, 2, 14-17.

Dubova, I., Apine, A., Grauda, G., Jankevica, L., 2020. Adaptation of techniques for testing antimicrobial properties of amber nano and micro size particles. 78th International Scientific Conference of the University of Latvia, Innovative and applied research in Biology, Proceedings, 2, 18-22.

Kruchonok, A., Strah, Y., Krasņevska, N., Anoshenko, B., Rashal, I., Butkauskas, D., Titok, V., Grauda, D., 2020. Populations of cloudberry (Rubus chamaemorus L.) as a potential source of antioxidants. 78th International Scientific Conference of the University of Latvia, Innovative and applied research in Biology, Proceedings, vol. 2, 27-30.

Stafecka I., Grauda D., Stramkale V., 2019., The evaluation of disease resistance of flax genotypes in relation to environmental factors. Zemdirbyste-Agriculture, 106 (4), 367–376. DOI 10.13080/z-a.2019.106.047.

Butkauskas D., Pilinkovskij A., Ragauskas A., Kesminas V., Fopp-Bayat D., 2019. Genetic characterization of Atlantic sturgeon stocking material used in Lithuania to restore the Baltic sea population. Acta Ichthyologica et Piscatoria, 49 (3): 251-256. DOI: 10.3750/AIEP/02557

Lyashenko I., Arta Viļuma-Gudmona, Grauda D., 2018. Mechanical Properties and Technological Features of Production of the Synthetic Juniper Fiber. IOSR Journal of Polymer and Textile Engineering Vol. 5, Issue 2, p.1-7., URL: iosrjournals.org/iosr-jpte/papers/Vol5-Issue2/A05020107.pdf ISSN 2348-0181.

Inga Lyashenko, Arta Viļuma-Gudmona, Dace Grauda, Julija Rombovska 2018. UV Protection Finishing of Natural and Blend Textiles Using Succinite Microparticles, AASCIT Journal of Health Number: 9720796 Vol.5, No.3, www.aascit.org/journal/archive2

KRASŅEVSKA, N., GRAUDA, D., KĻAVIŅA, D. and RASHAL, I., 2017. Endopolyploidy of endangered plant species Ligularia sibirica in different environments, Vide. Tehnologija. Resursi - Environment, Technology, Resources, 2017, pp. 161-164.

STAFECKA, I., GRAUDA, D. and STRAMKALE, V., 2017. Diseases diversity for flax genetic resources in Latvia, Vide. Tehnologija. Resursi - Environment, Technology, Resources 2017, pp. 278-283.

STAFECKA, I., STRAMKALE, V. and GRAUDA, D., 2016. Estimation of yield stbility for flax genetic resource using regression and cluster analysis. ReseaRch foR RuRal Development, 1. Book Series: Research for Rural Development. p. 15-22. 

GRAUDA, D., BUMBURE, L., LYASHENKO, I., KATASHEV, A., DEKHTYAR, Y. and RASHAL, I., 2015. Amber particles as living plant cell markers in flow cytometry. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 77-81.

 GRAUDA, D., KOLODYNSKI, A., BELOGRUDOVA, I., BUMBURE, L. and RASHAL, I., 2015. Combined effects of 50Hz electromagnetic field and SiO2 nanoparticles on oxidative stress in plant's gametic cells. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 82-86.

 GRAUDA, D., AVOTIŅŠ, K., FOKINA, O., KOLODINSKA-BRANTESTAM, A. and RASHAL, I., 2015. Genetic diversity of white clover (Trifolium Repens L.) from the Urban Area of Riga. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 132-134

 KOLODINSKA-BRANTESTAM, A., BOIKO, D., GRAUDA, D., KRASŅEVSKA, N. and RASHAL, I., 2015. Genetic diversity of mute swan population of the Riga Urban Area. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69(3), pp. 135-139.

 GRAUDA, D. and ČEKSTERE, G., 2015. Current investigations of urban enviromental quality in Latvia. Proceedings of the Latvian Academy of Sciences. Section B, 69(3), pl. A1.

 CEKSTERE, G., KARLSONS, A. and GRAUDA, D., 2015. Salinity-induced responses and resistance in Trifolium repens L. Urban Forestry and Urban Greening, 14(2), pp. 225-236.

 RAGAUSKAS, A., BUTKAUSKAS, D., SRUOGA, A., KESMINAS, V., RASHAL,  and  TZENG, W.N., 2014. Analysis of the genetic structure of the European eel Anguilla anguilla using the mtDNA D-loop region molecular marker. Fisheries Science, 80(3), pp. 463-474.

 KOLODINSKA BRANTESTAM, A., LEGZDINA, L., CRISTENSEN, T., WEIBULL, J., BOTHMER, R., MARTINOW, YNGAARD, N. and RASHAL, l. (2014) Characterisation of agronomic performance of Baltic spring barley material. Proceedings of the Latvian Academy of Sciences. Section B, 68(3/4), pp. 119–132.

 LEPSE, L., RASHAL, I. and  AKA-KACAR Y., 2014. Assessment of genetic variability between inbred and siblines of Latvian origin cucumber population using RAPD markers. G. Sarkisyan (Ed.) Proc. Eurasian Symposium on Vegetables and Greens. Acta Horticulturae, 1033, pp. 31-38.

 GRAUDA, D., MIKELSONE, A., ĻISINA, N., ŽAGATA, K., ORNICANS, R., FOKINA, O., LAPINA, L. and RASHAL, I. (2014). Anther Culture Effectiveness in Producing Doubled Haploids of Cereals. Proceedings of the Latvian Academy of Sciences. Sect. B,68(3/4), pp. 142-147.

 ROZE, D., JAKOBSONE, G., MEGRE, D.,  BELOGRUDOVA, I. and KARLOVSKA, A. 2014. Survival of Liparis loeselii (L.) as an early successional species in Engure region described based on ecological pecularities during the annual cycle. Proceedings of the Latvian Academy of Sciences, Section B, 68(1/2), pp.93-100.

 KĻAVIŅA, D., GRAUDA, D., PRIEDE, A. and RASHAL, I., 2014. Habitat diversity and genetic variability of Cypripedium calceolus in Latvia. Proceedings of 6th Planta Europa Conference, Committee on Nature Conservation, Polish Academy of Sciences, Kraków, pp. 91–97. 

 RAGAUSKAS, A. BUTKAUSKAS, D. SRUOGA,  A. KESMINAS, V. RASHAL, I. and TZENG, WN. 2014. Analysis of the genetic structure of the European eel Anguilla anguilla using the mtDNA D-loop region molecular marker. Fisheries Science, 80(3), pp. 463-474.

 MIKELSONE, A., GRAUDA, D., STRAMKALE, V., ORNICANS, R. and RASHAL, I. 2013. Using anther culture methood for flax breeding intensification. Environment, Technology, Resorces. (1), pp. 149-153.

 GRAUDA, D. MIKELSONE, A. AUZINA, A. STRAMKALE, V. and  RASHAL, I. (2013) Use of Plant Biotechnology Methods for flax breeding in Latvia. In: Zaikov G. E., Pudel F., eds. Organic Chemistry, Biochemistry, Biotechnology and Renewable Resources. Research and Development. Vol. 1. Today and Tomorrow. Nova Science Publishers, Inc., USA,  pp. 1-10.

 GRAUDA, D., LAPINA, L., JANSONE, B., JANSONS, A. and RASHAL, I., 2013. Recovering genetic resources of some legume species of Latvian origin by plant tissue culture. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 67(3), pp. 224-228.

 BLEIDERE, M.  GRUNTE, I. GRAUDA, D. and RASHAL, l., 2012. Morphological and agronomic characterizion of spring barley variety ‘Austris’., Acta Biol. Univ. Daugavpiliensis, 12(1), pp  44-50.

 BELOGRUDOVA, I. GRAUDA, D. JAKOBSONE, G. and  RRASHAL, I., 2012. Usability of retrotransposone-based molecular marker system to assess genetic diversity of Liparis loeselii., Acta Biol. Univ. Daugavpiliensis, 12(1),  pp 40-43.

 LAPIŅA,  L. GRAUDA, D. and RASHAL, I., 2012. Using retrotransposon-based molecular markers for analysis of genetic variability in Latvian populations of alfalfa. Acta Biol. Univ. Daugavpiliensis, 12(1), pp 84-88.

 KOLODINSKA BRANTESTAM, A. RASHAL, I. TUVESSON, S. WEIBULL, J.and  R. VON BOTHMER., 2012. Genetic profiles and diversity of baltic spring barley material., Proc.Latvian Acad. Sci,, Sect.B, 66(1/2), pp  10-20.

 KOKINA, I. and  RASHAL, I., 2012. Results of monitoring of the population of Blumeria graminis f.sp. hordei in Latvia in 2009-2010., Proc. Latvian Acad.Sci., Sect. B,  66(1/2), pp  41-47.

 BUTKAUSKAS, D. RAGAUSKAS, A. SRUOGA, A. KESMINAS, V. LOŽYS, L.  RASHAL, I. TZENG, W.-N. and ŽALAKEVIČIUS, M., 2012. Investigations into genetic diversity of the perch inhabiting Ignalina nuclear power plant cooler and other inland water bodies of Lithuania on the basis of mtDNA analysis., Veterinarija ir Zootchnika,  60(82), 7-15.

 KOKINA, I. SĻEDEVSKIS, Ē. GERBREDERS, V. GRAUDA, D. JERMAĻONOKA, M. VALAINE, K. GAVARĀNE, I. PIGŅIKA, I. FILIPOVIČS, M. and RASHAL, I., 2012. Reaction of flax (Linum usitatissimum L.) calli culture to supplement of medium by carbon nanoparticles. Proc. Latvian Acad. Sci., Sect. B, 66(4/5), pp 200-209.

 KĻAVIŅA, D. GRAUDA, D. PRIEDE, A. and RASHAL, I., 2011. A complex investigation of Cypripedium calceolus populations in Latvia. Planta Europa, pp 10.

 

MIKELSONE, A., GRAUDA, D., STRAMKALE, V. and RASHAL, I., 2011. Breeding for organic farming: Obtaining and evaluation of flax somaclonal families, Vide. Tehnologija. Resursi - Environment, Technology, Resources2011, pp. 231-236.

 LACIS, G., RASHAL, I. and TRAJKOVSKI, V., 2011. Implementation of a limited set of SSR markers for screening of genetic variability in Latvian and Swedish sour cherry (Prunus cerasus L.) genetic resources collections.Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 65(1-2), pp. 21-28.

 GRAUDA, D., STRAMKALE, V., KOMLAJEVA, Ļ., BRATESTAM, A.K., MIĶELSONE, A., LAPIŅA, L., AUZIŅA, A. and RASHAL, I., 2009. Evaluation of the Latvian flax genetic resources and perspective of their utilisation, Vide. Tehnologija. Resursi - Environment, Technology, Resources 2009, pp. 160-165.

LACIS, G., RASHAL, I., RUISA, S., TRAJKOVSKI, V. and IEZZONI, A.F., 2009. Assessment of genetic diversity of Latvian and Swedish sweet cherry (Prunus avium L.) genetic resources collections by using SSR (microsatellite) markers. Scientia Horticulturae, 121(4), pp. 451-457.

LACIS, G., RASHAL, I. and TRAJKOVSKI, V., 2010. Comparative analysis of sweet cherry (P. avium) genetic diversity revealed by two methods of SSR marker detection. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 64(3-4), pp. 149-158.

SRUOGA, A., RASHAL, I., BUTKAUSKAS, D., VIKSNE, J., ŽALAKEVIČIUS, M., ŠVAŽAS, S. and KAMINSKAITE, M., 2009. Haplotypic variability and population genetic structure of Garganey Anas querquedula and common pochard Aythyla ferina in the Western Palearctic. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 63(3), pp. 104-110.

GRAUDA, D., MIĶELSONE, A. and RASHAL, I., 2009. Use of antioxidants for enhancing flax multiplication rate in tissue culture.

JACQUARD, C., NOLIN, F., HÉCART, C., GRAUDA, D., RASHAL, I., DHONDT-CORDELIER, S., SANGWAN, R.S., DEVAUX, P., MAZEYRAT-GOURBEYRE, F. and CLÉMENT, C., 2009. Microspore embryogenesis and programmed cell death in barley: Effects of copper on albinism in recalcitrant cultivars. Plant Cell Reports, 28(9), pp. 1329-1339.

LAPIŅA, L., GRAUDA, D., JANSONE, B., JANSONS, A. and RASHAL, I., 2009. Restoration of latvian alfalfa (Medicago sativa) genetic resources perspective for breeding, Vide. Tehnologija. Resursi - Environment, Technology, Resources 2009, pp. 166-168.

Patents

Dace Grauda, ​​Aleksejs Katasevs, Lada Bumbure. - Non-destructive, fast flow cytometry method for bioindication (LV Patent Office No. 15171, September 29, 2017).

Registered varieties

Registration certificate No.373. The certificate was registered and issued by the State Plant Protection Service of the Ministry of Agriculture of the Republic of Latvia on November 24, 2009. In cooperation with the Plant Genetics Laboratory of the Institute of Biology of the University of Latvia and the State Stende Cereal Breeding Institute, using plant biotechnology - double haploid method, a new summer barley variety “AUSTRIS” has been selected and registered in Latvia. Authors: Sofija Kaliņina, Dace Grauda, ​​acad. Isaac Rašals, Māra Bleidere, Ilze Grunte, Solveiga Maļecka.

Registration certificate no. 410. The certificate was registered and issued by the State Plant Protection Service of the Ministry of Agriculture of the Republic of Latvia on 12 May 2011. In cooperation with the State Stende Cereal Breeding Institute, a new summer wheat variety "ROBIJS" has been created. Authors: Dace Grauda, ​​akad. Isaac Rashel, Vija Strazdina, Maija Ceraukste.