Laboratory of Experimental Entomology and Microbiology

ADDRESS: O.Vācieša str. 4, Rīga,  Latvija, LV-1004

Phone: +371 26183323,

E-Mail: liga.jankevica@lu.lv; exp-biology@inbox.lv

HEAD OF LABORATORY: Dr.biol. Līga Jankevica   

Staff:

Dr. biol. I. Salmane, Dr. biol. D. Teļnovs, Dr. biol. I. Zariņš (consulting) , M. Sc. Z. Metla &  M. Sc. I. Dubova, student A. Apine

Main research topics:

Biological control (fundamental and practical aspects)
1) Investigation of the prevalence of functional biodiversity (entomophages, parasitoids, acarophages, entomopathogenic fungi, bacteria, baculoviruses) in agrocenoses; 2) Optimization of biological plant protection methods and development of new biological plant protection preparations to limit the multiplication of the most important agricultural and forestry pests; 3) Isolation of new isolates of virulent entomopathogenic microorganisms, determination of their properties; 4) Development of environmentally friendly phytopreparations (insecticidal and fungicidal properties of various plant extracts, including conifers, as well as plant growth stimulating effects have been studied).

Studies on the prevalence and incidence of major plant pests and their natural enemies. Investigation of strawberry-associated phytophagous pests and their natural enemies. Studies on the distribution, biology and natural enemies of horse chestnut stripe. Research on rare and invasive invertebrates.

Plant - pest - parasitoid / predator or microorganism interactions
Investigation of natural enemies of various species of pests (parasitic and predatory arthropods, entomopathogenic microorganisms and viruses). Studies have been performed to elucidate the interaction of various microorganisms and pests at the cell, individual and population level. Based on the results of theoretical research, a methodology for the identification of entomopathogenic microorganisms (baculoviruses, bacteria, fungi) has been developed.

Environmental microbiology and biotechnology
Methods for detecting the presence of entomopathogenic viruses and fungi in the environment have been developed and tested. Approved methodology for soil health and quality assessment in urban environment by determining the biomass of microorganisms, the total number of microorganisms and the predominant species of microorganisms. Mastered and optimized methods for testing antimicrobial properties of nanoparticles and biotextiles.

Experience, competence and services:

  • selection and multiplication of biological agents (bacteria, fungi, insect viruses, acarophages and entomophagous organisms);
  • evaluation of the effectiveness and suitability of environmentally friendly plant protection products for use in plant protection;
  • evaluation of biological properties (fungicidal, insecticidal and bactericidal activity) of compounds produced by plants, plant extracts, phytopreparations and other products used in plant protection;
  • biological and microbiological quality assessment of various substrates (soil, peat, sapropel, humus, compost, wood by-products, textiles) and products (food, cosmetics, textiles, etc.).
  • Determination of biodegradation and resistance of biotextiles.

Methods and equipment for our services:

The laboratory is equipped with the necessary equipment for long-term vegetation testing, insect culture maintenance and bioassays. Traditional microbiological methods (isolation and propagation of microorganisms) using standard, modified and selective media, biochemical identification of microorganisms, soil biochemical testing (respiration, microorganism biomass, enzymatic activity, etc.), soil (water) toxicity assessment using germination test, FaxJaz

International cooperation and networking projects

Partners

  • State Scientific Research Institute Nature Research Center, abbreviated (NRC) (Vilnius, Lithuania) - cooperation in the IFSITEX project.
  • Company AB LINAS (Lithuania) - cooperation in the IFSITEX project.
  • Swiss Federal Technical University (ETH Zurich) - project "New approaches for the biological control of forest insect pests" .2013-2016. SCIEX project and development of doctoral thesis.
  • Siedlce University of Natural and Human Sciences, Department of Plant Protection, (Siedlce, Poland) - project "Investigations on occurrence and species diversity of acaropathogenic and entomopathogenic fungi in Latvia" - exchange visits Cezary Tkachuk and Zane Metla.
  • University of Copenhagen, Department of Ecology and Molecular Biology (Copenhagen, Denmark); application of entamopathogenic microorganisms in biological control, exchange of experience Jankevica.
  • Institute for Biological Control, Julius Kűhn Institute (Darmstadt, Germany); identification of entomopathogenic microorganisms and viruses, exchange of experience - Jankevica, Metla.

Research projects:

  • Sampling and testing of mollusc species (Mollusca). Nature Protection Board, head L. Jankevica, 2019 - 2021. Aim to obtain information and evaluate species diversity in protected areas in Latvia.

  • LU research project: "Biodiversity - impact, functions and protection". Subproject: “Biological research of living organisms in urban, rural and aquatic ecosystems”, 2016-2020. Aim - to evaluate plant pest populations and their natural enemies in urban green areas.

  • "The role of microorganism associations in the development and biological control of Lepidoptera forest pest pathogens" - doctoral thesis - Zane Metla (2012-2020). Aim - to study the interaction of associations of microorganisms of the middle intestinal microflora of forest pests and based on the obtained results, and to develop a new biological control method based on the synergistic relationship of microorganisms

Implemented projects

  • ESF, activity 1.1.1.2. “Attracting human resources to science”, project “Establishment of a new scientific group for urban natural environment quality research” No. 2013/0060 / 1DP / 1.1.1.2.0 / 13 / APIA / VIAA / 041 (Scientific supervisors Gunta Čekstere and Dace Grauda). 2013-2015. g.  The task of the laboratory was to study the micro-biological quality of soil in grasslands and urban forests in different territories of Riga. An impact assessment of the urbanization load in the urban environment has been performed; developed soil quality assessment methods tested in the urban environment.

  • ERDF project (No. 2010/0249 / 2DP / 2.1.1.2.0 / 10 / APIA / VIAA / 168) "Development of environmentally friendly plant protection products of plant origin based on coniferous biomass extracts". Project leader: Dr. silv. M. Daugavietis (SILAVA),   LU group leader Dr. biol. L. Jankevica. 2010-2013. A recipe and production method for an environmentally friendly plant protection product has been developed. In vitro tests have been performed on plant pathogens such as Botrytis cinerea, Colletotrichum acutatum, Phytophthora cactorum and Mycosphaerella fragariae.

  • Latvian Science Consil (LZP) project No. 09. 1549 "Biological plant protection, conservation of biological diversity and functional significance of plant mineral nutrition in globally changing environmental conditions" (2010-2012). Project manager: Dr.biol. Anita Osvalde.

  • LZP project No. 09.1586 "Interaction of plant mineral nutrients, qualitative and quantitative indicators of tree biomass, diseases and pests" (2010-2012) Project manager: Dr. silv. Maris Daugavietis.

  • LZP Project No. 09.1359 "Ecological risk and perspective of entomopathogenic toxin - forming bacteria in biological plant protection in Latvia". 2009. Head: Dr. biol. Līga Jankevica.

  • LZP project No. 09.1360 "Investigation of Insect Pathogen Dispersion and Transmission Mechanisms in Forest Pest Populations" 2009. Project manager: Dr.biol. Līga Jankevica.

  • LZP project No. 05.1411 “Investigation of the mechanisms of action of phytopreparations on various harmful organisms in crop biocenoses and determination of their spectrum of action” supervisor Dr.biol. Zariņš 2005–2008.

  • LZP project No. 05.1438 “Ecology of entomopathogenic microorganisms: distribution, genetic diversity and ecological host range. Risk assessment studies.” supervisor Dr.biol. Līga Jankevica. 1.01.2005 - 31.12.2008

  • LZP project No. 06.1942 “Biodiversity: key pests and their antagonists on blubeeries” Head Dr.biol. Čudare Z. 1.01.2006. - 31.12.2008

  • Investigation and elaboration of technologies for grooving entomophagous and acarophagous; their application in biological control.
  • Development of biopreparation “Fitoekols-IF” and “Fitorodents” on the basis of resin-acids extracted from pine and spruce needles, estimation an ecological and economical effectiveness.
  • Preservation and maintenance collection of entomopathogenic microorganisms and baculoviruses – environmental potential for providing plant protection
   

JANKEVICA L., TELNOV D., SALMANE I, ZIEMELE S., CEKSTERE G. (2019). Studies on soil microflora and microarthropod communities as soil health and quality indicators in urban forests in Rīga, Latvia. In: Conference Proceedings of 19th International Multidisciplinary Scientific Geoconference SGEM 2019, 30 June - 6 July. Water Resources. Forest, Marine and Ocean Ecosystems (Soils, Forest Ecosystems), Vol. 19, Issue: 3.2, 821-828. Q4

JANKEVICA L., MINOVA S., METLA Z., DAUGAVIETIS M. (2018). Development of new environmentally natural insecticides product from coniferous biomass agains insect pests. In: 18th International Multidisciplinary Scientific GeoConference, SGEM 2018, Conference Proceedings: Ecology, Economics, Education and Legislation, 18(5.1) 251-258.  Q4

JANKEVICA L., POLIS O., KORICA A., SAMSONE I., LAUGALE V. & DAUGAVIETIS, M. (2018) Environmental risk assessment studies on new plant protection products which have been elaborated from coniferous tree bark. Agronomy research. http://dx.doi.org/10.15159/ar.18.189

KRAMS I., KECKO S., INASHKINA I. .... JANKEVICA L., MEIJA L., KRAMA T. (2017). Food quality affects the expression of antimicrobial peptide genes upon simulated parasite attack in the larvae of greater wax moth. Entomologia Experimentalis et Applicata, 165, (2-3). Pages 129-137.

FLURY P., VESGA P., PÉCHY-TARR M., AELLEN N., DENNERT F., HOHER N., KUPFER-SCHMIED K.P., KUPFERSCHMIED P., METLA Z., …KEEL C.J. and MAURHOFER M., 2017. Antimicrobial and insecticidal: Cyclic lipopeptides and hydrogen cyanide produced by plant-beneficial Pseudomonas strains CHA0, CMR12a, and PCL1391 contribute to insect killing.  Frontiers in Microbiology,8, (art. no. 100). DOI: 10.3389/fmicb.2017.00100

FLURY P., AELLEN N., RUFFNERB., PéCHY-TARR M., FATAAR S., METLA Z.  … KEEL C. and MAURHOFER M., 2016. Insect pathogenicity in plant-beneficial pseudomonads: Phylogenetic distribution and comparative genomics. ISME Journal, 10 (10), pp. 2527-2542.

MINOVA S., JANKEVICA L., SALMANE I., CEKSTERE G. (2015). Preliminary Studies on Microbial Biomass and the Microarthropod Community as Soil Health and Quality Indicators in Urban Grasslands, Rīga as an Example. Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences 69(3):140-144, DOI: 10.1515/prolas-2015-0020

MINOVA S., SEŠĶĒNA R., VOITKĀNE S., METLA Z., DAUGAVIETIS M., JANKEVICA L. (2015). Impact of pine (Pinus sylvestris L.) and spruce (Picea abies (L.) Karst.) bark extracts on important strawberry pathogens, Proceeding of Latvian Academy of Science. Section B. Natural, Exact, and Applied Sciences. 69 (1-2): 62–67, DOI: 10.1515/prolas-2015-0008

SALMANE I., CIEMATNIEKS R., OZOLINA-POLE L., RALLE B., IEVINSH G. (2015). Investigation of European shot-hole borer, Xyleborus dispar (Coleoptera, Scolytidae), in apple orchards of Latvia. Proceedings of the 10th International Scientific and Practical Conference “Environment. Technology. Resources”, Rēzekne. Volume II: 256-260

VOLKOVA J., LAUGALE V., LEPSE L., BAŽENOVA A., JANKEVICA L., DAUGAVIETIS M. (2014). Evaluation of spruce biomass extract for control of grey mould (Botrytis cinerea) in field-grown strawberries. Environmental and Experimental Biology 12: 89–93.

JANKEVICA L., SAMSONE I., MINOVA S., SESKENA R., HALIMONA J., METLA Z., LAUGALE V., RANCANE R., DAUGAVIETIS M., ZARINS I. (2013). Elaboration of new environmentally friendly plant protection product from coniferous trees biomass against plant diseases. SGEM2013 Conference Proceedings, DOI: 10.5593/SGEM2013/BE5.V1/S20.047.

LAUGALE V., JANKEVICA L., SAMSONE I., HALIMONA J., METLA Z., LEPSIS J., DAUGAVIETIS M., RANCĀNE R. (2013). Preliminary studies on development of a new environmentally friendly plant protection product against grey mould.Proceeding of Latvian Academy of Science. Section B, Vol. 67, No. 2 (683), pp. 198-202. DOI:10.2478/prolas-2013-0033.

METLA Z., VOITKĀNE S., SEŠĶĒNA R., PETROVA V., JANKEVICA L. (2013). Presence of entomopathogenous fungi and bacteria in Latvian population of horse-chesnuth miner Cameraria ohridellaActa Biol. Univ. Daugavp. 13 (1):81-88.

PETROVA V., JANKEVICA,  L. SAMSONE, I., 2013. Composition of phytophagous insects associated with strawberry. Latvia. Proceeding of Latvian Academy of Science, Section B. 67(2), pp. 124-129.

JANKEVICA L., HALIMONA J., METLA Z., SESKENA R., ZARINS I. (2011). Investigation of nucleopolyhedroviruses and experience of regulation of forest pest populations in Latvia. IOBC/WPRS Bulletin, 66: 457-460.

PETROVA V., SAMSONE I., JANKEVICA L., ČUDARE Z. (2008) The predatory mite Neoseiulus cucumeris for control of the strawberry mite Phytonemus pallidus on strawberry runner plants. Proceedings of international scientific conference„ Sustainable Fruit Growing: From Plant To Product” Jurmala - Dobele, May 28-31, 2008, 250 -256.

SESKENA R., PETROVA V., JANKEVICA M., JANKEVICA L. (2008). Studies on ecological and physiological host range of entomopathogenic Hyphomycetes. IOBC/WPRS Bulletin, 31 (1) 198-203.  

HAĻIMONA J., JANKEVICA L. (2007). An experimental comparison of the virulence of fungal (Entomophtorales) isolates on Schizaphis graminum. Latvijas entomologs, 44: 95-102.

JANKEVICA L. (2004). Ecological associations between entomopathogenic fungi and pest insects recorded in Latvia. Latvijas Entomologs, 41: 60-65.

KRUZMANE D., JANKEVICA L. IEVINSH G. (2002). Effect of regurgitant from Leptinotarsa decemlineata Say on wound responses in Solanum tuberosum L. and Phaseolus vulgaris L. plants with special emphasis on ethylene production. Physiologia Plantarum, 115 (4) 577-585.

JANKEVICA L., CUDARE Z., OSE V. (1998). New isolate of Malacosoma neustria nuclear polyhedrosis virus in Latvia. Journal of Invertebrate Pathology. Vol. 71 (3) 283-285.

CUDARE Z. &  JANKEVICA L. (1997). The role of active plant extracts and granulosis viruses in regulation of pest populations. Proceedings of the Latvian Academy of Sciences, Section B Natural Exact and Applied Sciences. 51 (3-4):70-175

DAUGAVIETIS M,  POLIS O., KORICA A., JANKEVICA L., BARTKEVICS V., LEPSE L., RANCANE R. Biologically active composition inhibiting Botrytis cinerea infection and a method for producing it.  Patent.  LV14804 B, 20.05.2014.

ZARIŅŠ, I., EGLĪTE, G. 1994. Fitopreparāts augu sēnīšu slimību apkarošanai. Patents Nr.10559.IV